Rank |
Authors |
Title |
Journal |
Volume |
Pages |
Year |
Times cited |
1 |
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. |
Protein measurement with the folin phenol reagent. |
J. Biol. Chem. |
193 |
265–275 |
1951 |
305148 |
2 |
Laemmli, U. K. |
Cleavage of structural proteins during the assembly of the head of bacteriophage T4. |
Nature |
227 |
680–685 |
1970 |
213005 |
3 |
Bradford, M. M. |
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. |
Anal. Biochem. |
72 |
248–254 |
1976 |
155530 |
4 |
Sanger. F., Nicklen, S. & Couslon, A. R. |
DNA sequencing with chain-terminating inhibitors. |
Proc. Natl Acad. Sci. USA |
74 |
5463–5467 |
1977 |
65335 |
5 |
Chomczynski, P. & Sacchi, N. |
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. |
Anal. Biochem. |
162 |
156–159 |
1987 |
60397 |
6 |
Towbin, H., Staehelin, T. & Gordon, J. |
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. |
Proc. Natl Acad. Sci. USA |
76 |
4350–4354 |
1979 |
53349 |
7 |
Lee. C., Yang, W. & Parr, R. G. |
Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density. |
Phys. Rev. B |
37 |
785–789 |
1988 |
46702 |
8 |
Becke, A. D. |
Density-functional thermochemistry. III. The role of exact exchange. |
J. Chem. Phys. |
98 |
5648–5652 |
1993 |
46145 |
9 |
Folch, J., Lees, M. & Stanley, G. H. S. |
A simple method for the isolation and purification of total lipides from animal tissues. |
J. Biol. Chem. |
226 |
497–509 |
1957 |
45131 |
10 |
Thompson, J. D., Higgins, D. G. & Gibson, T. J |
Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. |
Nucleic Acids Res. |
22 |
4673–4680 |
1994 |
40289 |
11 |
Kaplan, E. L. & Meier, P. |
Nonparametric estimation from incomplete observations. |
J. Am. Stat. Assoc. |
53 |
457–481 |
1958 |
38600 |
12 |
Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. |
Basic local alignment search tool. |
J. Mol. Biol. |
215 |
403–410 |
1990 |
38380 |
13 |
Sheldrick, G. M. |
A short history of SHELX. |
Acta Crystallogr. A |
64 |
112–122 |
2008 |
37978 |
14 |
Altschul, S. F. et al. |
Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. |
Nucleic Acids Res. |
25 |
3389–3402 |
1997 |
36410 |
15 |
Murashige, T. & Skoog, F. |
A revised medium for rapid growth and bio assays with tobacco tissue cultures. |
Physiol. Plant. |
15 |
473–497 |
1962 |
36132 |
16 |
Perdew, J. P., Burke, K. & Ernzerhof, M. |
Generalized gradient approximation made simple. |
Phys. Rev. Lett. |
77 |
3865–3868 |
1996 |
35405 |
17 |
Folstein, M. F., Folstein, S. E. & McHugh, P. R. |
“Mini-mental state”: A practical method for grading cognitive state of patients for clinician. |
J. Psychiatr. Res. |
12 |
189–198 |
1975 |
34532 |
18 |
Bligh, E. G. & Dyer, W. J. |
A rapid method of total lipid extraction and purification. |
Can. J. Biochem. Physiol. |
37 |
911–917 |
1959 |
32131 |
19 |
Southern, E. M. |
Detection of specific sequences among DNA fragments separated by gel-electrophoresis. |
J. Mol. Biol. |
98 |
503 |
1975 |
31904 |
20 |
Saitou, N. & Nei, M. |
The neighbor-joining method: A new method for reconstructing phylogenetic trees. |
Mol. Biol. Evol. |
4 |
406–425 |
1987 |
30176 |
21 |
Livak, K. J. & Schmittgen, T. D. |
Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. |
Methods |
25 |
402–408 |
2001 |
28870 |
22 |
Shannon, R. D. |
Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides. |
Acta Crystallogr. A |
32 |
751–767 |
1976 |
28658 |
23 |
Otwinowski, Z. & Minor, W. |
Processing of X-ray diffraction data collected in oscillation mode. |
Method. Enzymol. A |
276 |
307–326 |
1997 |
28647 |
24 |
Cox, D. R. |
Regression models and life-tables. |
J. R. Stat. Soc., B |
34 |
187–220 |
1972 |
28439 |
25 |
Becke, A. D. |
Density-functional exchange-energy approximation with correct asymptotic-behavior. |
Phys. Rev. A |
38 |
3098–3100 |
1988 |
26475 |
26 |
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. |
Colorimetric method for determination of sugars and related substances. |
Anal. Chem. |
28 |
350–356 |
1956 |
25735 |
27 |
Reynolds, E. S. |
Use of lead citrate at high pH as an electron-opaque stain in electron microscopy. |
J. Cell Biol. |
17 |
208 |
1963 |
24449 |
28 |
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. |
The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. |
Nucleic Acids Res. |
25 |
4876–4882 |
1997 |
24098 |
29 |
Bland, J. M. & Altman, D. G. |
Statistical methods for assessing agreement between two methods of clinical measurement. |
Lancet |
327 |
307–310 |
1986 |
23826 |
30 |
Weber, K. & Osborn, M. |
Reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. |
J. Biol. Chem. |
244 |
4406 |
1969 |
23642 |
31 |
Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. |
Isolation of biologically-active ribonucleic-acid from sources enriched in ribonuclease. |
Biochemistry |
18 |
5294–5299 |
1979 |
23435 |
32 |
Scatchard, G. |
The attractions of proteins for small molecules and ions. |
Ann. New York Acad. Sci. |
51 |
660-672 |
1949 |
23421 |
33 |
Baron, R. M. & Kenny, D. A. |
The moderator–mediator variable distinction in social psychological-research — conceptual, strategic, and statistical considerations. |
J. Pers. Soc. Psychol. |
51 |
1173–1182 |
1986 |
23356 |
34 |
Kohn, W. & Sham, L. J. |
Self-consistent equations including exchange and correlation effects. |
Phys. Rev. |
140 |
A1133 |
1965 |
23059 |
35 |
Mosmann, T. |
Rapid colorimetric assay for cellular growth and survival — application to proliferation and cyto-toxicity assays. |
J. Immunol. Methods |
65 |
55–63 |
1983 |
23011 |
36 |
Iijima, S. |
Helical microtubules of graphitic carbon. |
Nature |
354 |
56–58 |
1991 |
22899 |
37 |
Fiske, C. H. & Subbarow, Y |
The colorimetric determination of phosphorus. |
J. Biol. Chem. |
66 |
375–400 |
1925 |
22690 |
38 |
Davis, B. J. |
Disc electrophoresis — II. Method and application to human serum proteins. |
Ann. New York Acad. Sci. |
121 |
404–427 |
1964 |
22074 |
39 |
Hohenberg, P. & Kohn, W. |
Inhomogeneous electron gas. |
Phys. Rev. B |
136 |
B864 |
1964 |
21931 |
40 |
Feinberg, A. P. & Vogelstein, B. |
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. |
Anal. Biochem. |
132 |
6–13 |
1983 |
21446 |
41 |
Felsenstein, J. |
Confidence limits on phylogenies: an approach using the bootstrap |
Evolution |
39 |
783–791 |
1985 |
21373 |
42 |
Grynkiewicz, G., Poenie, M. & Tsien, R. Y. |
A new generation of Ca2+ indicators with greatly improved fluorescence properties |
J. Biol. Chem. |
260 |
3440–3450 |
1985 |
19561 |
43 |
Kresse, G. & Furthmüller, J. |
Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set. |
Phys. Rev. B |
54 |
11169–11186 |
1996 |
18856 |
44 |
O’Farrell, P. H. |
High-resolution 2-dimensional electrophoresis of proteins. |
J. Biol. Chem. |
250 |
4007–4021 |
1975 |
18489 |
45 |
Tamura, K., Dudley, J., Nei, M. & Kumar, S. |
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. |
Mol. Biol. Evol. |
24 |
1596–1599 |
2007 |
18286 |
46 |
Zadeh, L. A. |
Fuzzy sets. |
Inform. Control |
8 |
338–353 |
1965 |
18203 |
47 |
Sheldrick. G. M. |
Phase annealing in SHELX-90: direct methods for larger structures. |
Acta Crystallogr. A |
46 |
467–473 |
1990 |
17728 |
48 |
McKhann, G. et al. |
Clinical diagnosis of Alzheimer’s disease: Report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer’s Disease. |
Neurology |
34 |
939–944 |
1984 |
17220 |
49 |
Monkhorst, H. J. & Pack, J. D |
Special points for Brillouin-zone integrations. |
Phys. Rev. B |
13 |
5188–5192 |
1976 |
17087 |
50 |
Burton, K. |
Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. |
Biochem. J. |
62 |
315-323 |
1956 |
17067 |
51 |
Radloff, L. S. |
The CES-D scale: a self-report depression scale for research in the general population. |
Appl. Psychol. Meas. |
1 |
385–401 |
1977 |
17055 |
52 |
Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. |
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. |
Pflug. Arch. Eur. J. Physiol. |
391 |
85–100 |
1981 |
17025 |
53 |
Hamilton, M. |
A rating scale for depression. |
J. Neurol. Neurosurg. Psychiatry |
23 |
56–62 |
1960 |
16734 |
54 |
Beck, A. T., Ward, C. H., Mendelsohn, M., Mock, J. & Erbaugh, J. |
An inventory for measuring depression. |
Arch. Gen. Psychiatry |
4 |
561 |
1961 |
16264 |
55 |
Kyte, J. & Doolittle, R. F. |
A simple method for displaying the hydropathic character of a protein. |
J. Mol. Biol. |
157 |
105–132 |
1982 |
16059 |
56 |
Gornall, A. G., Bardawill, C. J. & David, M. M. |
Determination of serum proteins by means of the biuret reaction. |
J. Biol. Chem. |
177 |
751–766 |
1949 |
16009 |
57 |
Dempster, A. P., Laird, N. M. & Rubin, D. B. |
Maximum likelihood from incomplete data via EM algorithm. |
J. R. Stat. Soc., B |
39 |
1–38 |
1977 |
15993 |
58 |
Metropolis, N., Rosenbluth, A. W., Rosenbluth, M. N., Teller, A. H. & Teller, E. |
Equation of state calculations by fast computing machines. |
J. Chem. Phys. |
21 |
1087–1092 |
1953 |
15902 |
59 |
Benjamini, Y. & Hochberg, Y. |
Controlling the false discovery rate: a practical and powerful approach to multiple testing. |
J. R. Stat. Soc. B |
57 |
289–300 |
1995 |
15898 |
60 |
Smith, P. K. et al. |
Measurement of protein using bicinchoninic acid. |
Anal. Biochem. |
150 |
76–85 |
1985 |
15802 |
61 |
Oldfield, R. C. |
The assessment and analysis of handedness: the Edinburgh inventory. |
Neuropsychologia |
9 |
97–113 |
1971 |
15517 |
62 |
Friedewald, W. T., Levy, R. I. & Fredrickson, D. S. |
Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. |
Clin. Chem. |
18 |
499 |
1972 |
15469 |
63 |
Saiki, R. K. et al. |
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. |
Science |
239 |
487–491 |
1988 |
15160 |
64 |
Duncan, D. B. |
Multiple range and multiple F tests. |
Biometrics |
11 |
1–42 |
1955 |
15047 |
65 |
Novoselov, K. S. et al. |
Electric field effect in atomically thin carbon films. |
Science |
306 |
666–669 |
2004 |
15022 |
66 |
Ellman, G. L. |
Tissue sulfhydryl groups. |
Arch. Biochem. Biophys. |
82 |
70–77 |
1959 |
15019 |
67 |
Böyum, A. |
Isolation of mononuclear cells and granulocytes from human blood. |
Scand. J. Clin. Lab. Invest. |
21 |
S77–S89 |
1968 |
14934 |
68 |
Landis, J. R. & Koch, G. G. |
The measurement of observer agreement for categorical data. |
Biometrics |
33 |
159–174 |
1977 |
14903 |
69 |
Brünger, A. T. et al. |
Crystallography & NMR system: a new software suite for macromolecular structure determination. |
Acta Crystallogr. D |
54 |
905–921 |
1998 |
14898 |
70 |
Dunning, T. H. Jr |
Gaussian-basis sets for use in correlated molecular calculations. 1. The atoms boron through neon and hydrogen. |
J. Chem. Phys. |
90 |
1007–1023 |
1989 |
14617 |
71 |
Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. |
PROCHECK: a program to check the stereochemical quality of protein structures. |
J. Appl. Crystallogr. |
26 |
283–291 |
1993 |
14462 |
72 |
Ware, J. E. Jr & Sherbourne, C. D. |
The MOS 36-item short-form health survey (SF-36): I. Conceptual framework and item selection. |
Med. Care |
30 |
473–483 |
1992 |
14332 |
73 |
Akaike, H. |
A new look at statistical-model identification. |
IEEE Trans. Automat. Contr. |
19 |
716–723 |
1974 |
14275 |
74 |
Yanisch-Perron, C., Vieira, J. & Messing, J. |
Improved M13 phage cloning vectors and host strains — nucleotide-sequences of the M13mp18 and pUC19 vectors |
Gene |
33 |
103–119 |
1985 |
14232 |
75 |
Devereux, J., Haeberli, P. & Smithies, O. |
A comprehensive set of sequence-analysis programs for the vax. |
Nucleic Acids Res. |
12 |
387–395 |
1984 |
14226 |
76 |
Posada, D. & Crandall, K. A. |
MODELTEST: Testing the model of DNA. |
Bioinformatics |
14 |
817–818 |
1998 |
14099 |
77 |
Kresse, G. & Joubert, D. |
From ultrasoft pseudopotentials to the projector augmented-wave method. |
Phys. Rev. B |
59 |
1758–1775 |
1999 |
14049 |
78 |
Hsu, S.-M., Raine, L. & Fanger, H. |
Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. |
J. Histochem. Cytochem. |
29 |
577–580 |
1981 |
13881 |
79 |
Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. |
Comparison of simple potential functions for simulating liquid water. |
J. Chem. Phys. |
79 |
926–935 |
1983 |
13774 |
80 |
Dewar, M. J. S., Zoebisch, E. G., Healy, E. F. & Stewart, J. J. P |
The development and use of quantum-mechanical molecular-models. 76. AM1: a new general-purpose quantum-mechanical molecular-model |
J. Am. Chem. Soc. |
107 |
3902–3909 |
1985 |
13718 |
81 |
Bartlett, G. R. |
Phosphorus assay in column chromatography. |
J. Biol. Chem. |
234 |
466–468 |
1959 |
13523 |
82 |
Kraulis, P. J. |
MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. |
J. Appl. Crystallogr. |
24 |
946–950 |
1991 |
13496 |
83 |
Bondi, A. |
Van der Waals volumes and radii. |
J. Phys. Chem. |
68 |
441–451 |
1964 |
13417 |
84 |
Ellman, G. L., Courtney, K. D., Andres, V. Jr & Featherstone, R. M. |
A new and rapid colorimetric determination of acetylcholinesterase activity. |
Biochem. Pharmacol. |
7 |
88 |
1961 |
13332 |
85 |
Blöchl, P. E |
Projector augmented-wave method. |
Phys. Rev. B |
50 |
17953–17979 |
1994 |
13330 |
86 |
Kirkpatrick. S., Gelatt, C. D. & Vecchi, M. P. |
Optimization by simulated annealing. |
Science |
220 |
671–680 |
1983 |
13293 |
87 |
Moncada, S., Palmer, R. M. & Higgs, E. A. |
Nitric oxide: physiology, pathophysiology, and pharmacology. |
Pharmacol. Rev. |
43 |
109–142 |
1991 |
13267 |
88 |
Marquardt, D. W. |
An algorithm for least-squares estimation of nonlinear parameters. |
J. Soc. Ind. Appl. Math. |
11 |
431–441 |
1963 |
13258 |
89 |
Kresse, G. & Furthmüller, J. |
Efficiency of ab initio total energy calculations for metals and semiconductors using a plane-wave basis set. |
Comput. Mater. Sci. |
6 |
15–50 |
1996 |
13084 |
90 |
O’Regan, B. & Grätzel, M. |
A low-cost, high-efficiency solar-cell based on dye-sensitized colloidal TiO2 films. |
Nature |
353 |
737–740 |
1991 |
12873 |
91 |
Spurr, A. R. |
A low-viscosity epoxy resin embedding medium for electron microscopy. |
J. Ultrastruct. Res. |
26 |
31 |
1969 |
12807 |
92 |
Berman, H. M. et al. |
The Protein Data Bank. |
Nucleic Acids Res. |
28 |
235–242 |
2000 |
12754 |
93 |
Perdew, J. P. & Wang, Y. |
Accurate and simple analytic representation of the electron-gas correlation-energy |
Phys. Rev. B |
45 |
13244–13249 |
1992 |
12748 |
94 |
Bimboim, H. C. & Doly, J. |
Rapid alkaline extraction procedure for screening recombinant plasmid DNA. |
Nucleic Acids Res. |
7 |
1513–1523 |
1979 |
12721 |
95 |
Jones, T. A., Zou, J.-Y., Cowan, S. W. & Kjeldgaard, M. |
Improved methods for building protein models in electron-density maps and the location of errors in these models. |
Acta Crystallogr. A |
47 |
110–119 |
1991 |
12649 |
96 |
Vosko, S. H., Wilk, L. & Nusair, M. |
Accurate spin-dependent electron liquid correlation energies for local spin-density calculations — a critical analysis. |
Can. J. Phys. |
58 |
1200–1211 |
1980 |
12583 |
97 |
Köhler, G. & Milstein, C. |
Continuous cultures of fused cells secreting antibody of predefined specificity. |
Nature |
256 |
495–497 |
1975 |
12391 |
98 |
Matthews, D. R. et al. |
Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. |
Diabetologia |
28 |
412-419 |
1985 |
12257 |
99 |
Brunauer, S., Emmett, P. H. & Teller, E. |
Adsorption of gases in multimolecular layers. |
J. Am. Chem. Soc. |
60 |
309–319 |
1938 |
12252 |
100 |
Ronquist, F. & Huelsenbeck, J. P. |
MrBayes 3: Bayesian phylogenetic inference under mixed models |
Bioinformatics |
19 |
1572-1574 |
2003 |
12209 |
101 |
Ross, R. |
Atherosclerosis — an inflammatory disease. |
N. Engl. J. Med. |
340 |
115–126 |
1999 |
12119 |