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100篇高引paper

The top 100 high cited papers

2014年Nature登了篇史上最高引100篇paper的文章 (Ref1: Nature 514, 550–553 (30 October 2014) doi:10.1038/514550a).史上最高引的是JBC上的Lowry蛋白定量法.涛哥和Becke的B3LYP都进入了前10了.以后按趋势很可能会成为前三呢 ╮(╯▽╰)╭ 看看过过瘾吧~

Paper Mountain

Rank Authors Title Journal Volume Pages Year Times cited
1 Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 265–275 1951 305148
2 Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685 1970 213005
3 Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 248–254 1976 155530
4 Sanger. F., Nicklen, S. & Couslon, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA 74 5463–5467 1977 65335
5 Chomczynski, P. & Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 156–159 1987 60397
6 Towbin, H., Staehelin, T. & Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci. USA 76 4350–4354 1979 53349
7 Lee. C., Yang, W. & Parr, R. G. Development of the Colle-Salvetti correlation-energy formula into a functional of the electron density. Phys. Rev. B 37 785–789 1988 46702
8 Becke, A. D. Density-functional thermochemistry. III. The role of exact exchange. J. Chem. Phys. 98 5648–5652 1993 46145
9 Folch, J., Lees, M. & Stanley, G. H. S. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226 497–509 1957 45131
10 Thompson, J. D., Higgins, D. G. & Gibson, T. J Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22 4673–4680 1994 40289
11 Kaplan, E. L. & Meier, P. Nonparametric estimation from incomplete observations. J. Am. Stat. Assoc. 53 457–481 1958 38600
12 Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. Basic local alignment search tool. J. Mol. Biol. 215 403–410 1990 38380
13 Sheldrick, G. M. A short history of SHELX. Acta Crystallogr. A 64 112–122 2008 37978
14 Altschul, S. F. et al. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25 3389–3402 1997 36410
15 Murashige, T. & Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15 473–497 1962 36132
16 Perdew, J. P., Burke, K. & Ernzerhof, M. Generalized gradient approximation made simple. Phys. Rev. Lett. 77 3865–3868 1996 35405
17 Folstein, M. F., Folstein, S. E. & McHugh, P. R. “Mini-mental state”: A practical method for grading cognitive state of patients for clinician. J. Psychiatr. Res. 12 189–198 1975 34532
18 Bligh, E. G. & Dyer, W. J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37 911–917 1959 32131
19 Southern, E. M. Detection of specific sequences among DNA fragments separated by gel-electrophoresis. J. Mol. Biol. 98 503 1975 31904
20 Saitou, N. & Nei, M. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 406–425 1987 30176
21 Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods 25 402–408 2001 28870
22 Shannon, R. D. Revised effective ionic radii and systematic studies of interatomic distances in halides and chalcogenides. Acta Crystallogr. A 32 751–767 1976 28658
23 Otwinowski, Z. & Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Method. Enzymol. A 276 307–326 1997 28647
24 Cox, D. R. Regression models and life-tables. J. R. Stat. Soc., B 34 187–220 1972 28439
25 Becke, A. D. Density-functional exchange-energy approximation with correct asymptotic-behavior. Phys. Rev. A 38 3098–3100 1988 26475
26 DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28 350–356 1956 25735
27 Reynolds, E. S. Use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17 208 1963 24449
28 Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. The CLUSTAL_X Windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25 4876–4882 1997 24098
29 Bland, J. M. & Altman, D. G. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 327 307–310 1986 23826
30 Weber, K. & Osborn, M. Reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244 4406 1969 23642
31 Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. Isolation of biologically-active ribonucleic-acid from sources enriched in ribonuclease. Biochemistry 18 5294–5299 1979 23435
32 Scatchard, G. The attractions of proteins for small molecules and ions. Ann. New York Acad. Sci. 51 660-672 1949 23421
33 Baron, R. M. & Kenny, D. A. The moderator–mediator variable distinction in social psychological-research — conceptual, strategic, and statistical considerations. J. Pers. Soc. Psychol. 51 1173–1182 1986 23356
34 Kohn, W. & Sham, L. J. Self-consistent equations including exchange and correlation effects. Phys. Rev. 140 A1133 1965 23059
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36 Iijima, S. Helical microtubules of graphitic carbon. Nature 354 56–58 1991 22899
37 Fiske, C. H. & Subbarow, Y The colorimetric determination of phosphorus. J. Biol. Chem. 66 375–400 1925 22690
38 Davis, B. J. Disc electrophoresis — II. Method and application to human serum proteins. Ann. New York Acad. Sci. 121 404–427 1964 22074
39 Hohenberg, P. & Kohn, W. Inhomogeneous electron gas. Phys. Rev. B 136 B864 1964 21931
40 Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132 6–13 1983 21446
41 Felsenstein, J. Confidence limits on phylogenies: an approach using the bootstrap Evolution 39 783–791 1985 21373
42 Grynkiewicz, G., Poenie, M. & Tsien, R. Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties J. Biol. Chem. 260 3440–3450 1985 19561
43 Kresse, G. & Furthmüller, J. Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set. Phys. Rev. B 54 11169–11186 1996 18856
44 O’Farrell, P. H. High-resolution 2-dimensional electrophoresis of proteins. J. Biol. Chem. 250 4007–4021 1975 18489
45 Tamura, K., Dudley, J., Nei, M. & Kumar, S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24 1596–1599 2007 18286
46 Zadeh, L. A. Fuzzy sets. Inform. Control 8 338–353 1965 18203
47 Sheldrick. G. M. Phase annealing in SHELX-90: direct methods for larger structures. Acta Crystallogr. A 46 467–473 1990 17728
48 McKhann, G. et al. Clinical diagnosis of Alzheimer’s disease: Report of the NINCDS-ADRDA Work Group under the auspices of Department of Health and Human Services Task Force on Alzheimer’s Disease. Neurology 34 939–944 1984 17220
49 Monkhorst, H. J. & Pack, J. D Special points for Brillouin-zone integrations. Phys. Rev. B 13 5188–5192 1976 17087
50 Burton, K. Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62 315-323 1956 17067
51 Radloff, L. S.  The CES-D scale: a self-report depression scale for research in the general population. Appl. Psychol. Meas. 1 385–401 1977 17055
52 Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflug. Arch. Eur. J. Physiol. 391 85–100 1981 17025
53 Hamilton, M. A rating scale for depression. J. Neurol. Neurosurg. Psychiatry 23 56–62 1960 16734
54 Beck, A. T., Ward, C. H., Mendelsohn, M., Mock, J. & Erbaugh, J. An inventory for measuring depression. Arch. Gen. Psychiatry 4 561 1961 16264
55 Kyte, J. & Doolittle, R. F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157 105–132 1982 16059
56 Gornall, A. G., Bardawill, C. J. & David, M. M. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 177 751–766 1949 16009
57 Dempster, A. P., Laird, N. M. & Rubin, D. B. Maximum likelihood from incomplete data via EM algorithm. J. R. Stat. Soc., B 39 1–38 1977 15993
58 Metropolis, N., Rosenbluth, A. W., Rosenbluth, M. N., Teller, A. H. & Teller, E. Equation of state calculations by fast computing machines. J. Chem. Phys. 21 1087–1092 1953 15902
59 Benjamini, Y. & Hochberg, Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J. R. Stat. Soc. B 57 289–300 1995 15898
60 Smith, P. K. et al. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150 76–85 1985 15802
61 Oldfield, R. C. The assessment and analysis of handedness: the Edinburgh inventory. Neuropsychologia 9 97–113 1971 15517
62 Friedewald, W. T., Levy, R. I. & Fredrickson, D. S. Estimation of concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin. Chem. 18 499 1972 15469
63 Saiki, R. K. et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239 487–491 1988 15160
64 Duncan, D. B. Multiple range and multiple F tests. Biometrics 11 1–42 1955 15047
65 Novoselov, K. S. et al. Electric field effect in atomically thin carbon films. Science 306 666–669 2004 15022
66 Ellman, G. L. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82 70–77 1959 15019
67 Böyum, A. Isolation of mononuclear cells and granulocytes from human blood. Scand. J. Clin. Lab. Invest. 21 S77–S89 1968 14934
68 Landis, J. R. & Koch, G. G. The measurement of observer agreement for categorical data. Biometrics 33 159–174 1977 14903
69 Brünger, A. T. et al. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr. D 54 905–921 1998 14898
70 Dunning, T. H. Jr Gaussian-basis sets for use in correlated molecular calculations. 1. The atoms boron through neon and hydrogen. J. Chem. Phys. 90 1007–1023 1989 14617
71 Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallogr. 26 283–291 1993 14462
72 Ware, J. E. Jr & Sherbourne, C. D. The MOS 36-item short-form health survey (SF-36): I. Conceptual framework and item selection. Med. Care 30 473–483 1992 14332
73 Akaike, H. A new look at statistical-model identification. IEEE Trans. Automat. Contr. 19 716–723 1974 14275
74 Yanisch-Perron, C., Vieira, J. & Messing, J. Improved M13 phage cloning vectors and host strains — nucleotide-sequences of the M13mp18 and pUC19 vectors Gene 33 103–119 1985 14232
75 Devereux, J., Haeberli, P. & Smithies, O. A comprehensive set of sequence-analysis programs for the vax. Nucleic Acids Res. 12 387–395 1984 14226
76 Posada, D. & Crandall, K. A. MODELTEST: Testing the model of DNA. Bioinformatics 14 817–818 1998 14099
77 Kresse, G. & Joubert, D. From ultrasoft pseudopotentials to the projector augmented-wave method. Phys. Rev. B 59 1758–1775 1999 14049
78 Hsu, S.-M., Raine, L. & Fanger, H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29 577–580 1981 13881
79 Jorgensen, W. L., Chandrasekhar, J., Madura, J. D., Impey, R. W. & Klein, M. L. Comparison of simple potential functions for simulating liquid water. J. Chem. Phys. 79 926–935 1983 13774
80 Dewar, M. J. S., Zoebisch, E. G., Healy, E. F. & Stewart, J. J. P The development and use of quantum-mechanical molecular-models. 76. AM1: a new general-purpose quantum-mechanical molecular-model J. Am. Chem. Soc. 107 3902–3909 1985 13718
81 Bartlett, G. R. Phosphorus assay in column chromatography. J. Biol. Chem. 234 466–468 1959 13523
82 Kraulis, P. J. MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallogr. 24 946–950 1991 13496
83 Bondi, A. Van der Waals volumes and radii. J. Phys. Chem. 68 441–451 1964 13417
84 Ellman, G. L., Courtney, K. D., Andres, V. Jr & Featherstone, R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7 88 1961 13332
85 Blöchl, P. E Projector augmented-wave method. Phys. Rev. B 50 17953–17979 1994 13330
86 Kirkpatrick. S., Gelatt, C. D. & Vecchi, M. P. Optimization by simulated annealing. Science 220 671–680 1983 13293
87 Moncada, S., Palmer, R. M. & Higgs, E. A. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43 109–142 1991 13267
88 Marquardt, D. W. An algorithm for least-squares estimation of nonlinear parameters. J. Soc. Ind. Appl. Math. 11 431–441 1963 13258
89 Kresse, G. & Furthmüller, J. Efficiency of ab initio total energy calculations for metals and semiconductors using a plane-wave basis set. Comput. Mater. Sci. 6 15–50 1996 13084
90 O’Regan, B. & Grätzel, M. A low-cost, high-efficiency solar-cell based on dye-sensitized colloidal TiO2 films. Nature 353 737–740 1991 12873
91 Spurr, A. R. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26 31 1969 12807
92 Berman, H. M. et al. The Protein Data Bank. Nucleic Acids Res. 28 235–242 2000 12754
93 Perdew, J. P. & Wang, Y. Accurate and simple analytic representation of the electron-gas correlation-energy Phys. Rev. B 45 13244–13249 1992 12748
94 Bimboim, H. C. & Doly, J. Rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7 1513–1523 1979 12721
95 Jones, T. A., Zou, J.-Y., Cowan, S. W. & Kjeldgaard, M. Improved methods for building protein models in electron-density maps and the location of errors in these models. Acta Crystallogr. A 47 110–119 1991 12649
96 Vosko, S. H., Wilk, L. & Nusair, M. Accurate spin-dependent electron liquid correlation energies for local spin-density calculations — a critical analysis. Can. J. Phys. 58 1200–1211 1980 12583
97 Köhler, G. & Milstein, C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256 495–497 1975 12391
98 Matthews, D. R. et al. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28 412-419 1985 12257
99 Brunauer, S., Emmett, P. H. & Teller, E. Adsorption of gases in multimolecular layers. J. Am. Chem. Soc. 60 309–319 1938 12252
100 Ronquist, F. & Huelsenbeck, J. P. MrBayes 3: Bayesian phylogenetic inference under mixed models Bioinformatics 19 1572-1574 2003 12209
101 Ross, R. Atherosclerosis — an inflammatory disease. N. Engl. J. Med. 340 115–126 1999 12119

Reference

  1. The top 100 papers


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